HEPES, Low Metals
CAS No: 7365-45-9
Catalog No.: H7365LMG
Purity: ≥99.5%
| Size | Price |
|---|---|
| 100G | $195.00 |
| 250G | $375.00 |
| 500G | $495.00 |
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Technical Information
HEPES has been described as one of the best all-purpose buffers available for biological research.1 Similar to many amino acids, HEPES is zwitterionic at most biological pHs. It's effective as a buffer in the pH range of 6.8 to 8.2, and in concentrations between 10 millimolar to 25 millimolar. HEPES is one of the most effective buffers in the important physiological pH range of 7.2 to 7.6. HEPES has been utilized extensively for research and commercial applications in the cell culture and tissue culture industries. Other large application areas include cosmetics and skin care, electronics and computer chip manufacturing.
1 N.E. Good, et al., Biochemistry, 5, 467 (1966).
CAS Number 7365-45-9
Name HEPES Ultra Pure Grade
Synonyms 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid; N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid; 2-[4-(2-Hydroxyethyl)-1-piperazine]ethanesulfonic acid
Molecular Formula C8H18N2O4S
Molecular Weight 238.30
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Specifications
| Test | H7365LMG, Low Metals |
|---|---|
| Appearance | White crysatlline powder |
| Assay (by titration, on dried basis) | NLT 99.5% |
| Solubility (1M solution H2O) | Clear, complete and colorless |
| Identification (by IR) | Conforms to structure |
| Melting Point | -- |
| Water (105°C for 3 hours) | ≤1.0% |
| Water (KF) | ≤0.1% |
| pH (5% solution in water) | 5.0 - 6.5 |
| pKa (@25°C) | 7.45 - 7.65 |
| UV Absorption (1M @290nm) | ≤0.04 |
| UV Absorption (1M @280nm) | ≤0.05 |
| UV Absorption (1M @260nm) | ≤0.05 |
| Residue on Ignition | ≤0.1% |
| Insoluble Matter | -- |
| Heavy Metals (Lead) | ≤0.0005% |
Biological Impurity Testing
| Test | H7365LMG, Low Metals |
|---|---|
| Endotoxin | -- |
| Endotoxin (0.2% solution in H2O | -- |
| Bioburden | -- |
| RNase, DNase, Nickase, Protease | -- |
| Cell culture test | -- |
Trace Impurity Testing
| Aluminum (Al) | ≤ 0.0005% |
|---|---|
| Arsenic (As) | ≤ 0.0001% |
| Barium (Ba) | ≤ 0.0005% |
| Bismuth (Bi) | ≤ 0.0005% |
| Cadmium (Cd) | ≤ 0.0005% |
| Calcium (Ca) | ≤ 0.001% |
| Chloride (Cl) | ≤ 0.05% |
| Chromium (Cr) | ≤ 0.0005% |
| Cobalt (Co) | ≤ 0.0005% |
| Copper (Cu) | ≤ 0.0005% |
| Iron (Fe) | ≤0.0005% |
| Lithium (Li) | ≤ 0.0005% |
| Magnesium (Mg) | ≤ 0.0005% |
| Manganese (Mn) | ≤ 0.0005% |
| Molybdenium (Mo) | ≤ 0.0005% |
| Nickel (Ni) | ≤ 0.0005% |
| Potassium (K) | ≤ 0.05% |
| Sodium (Na) | ≤ 0.05% |
| Strontium (Sr) | ≤ 0.0005% |
| Zinc (Zn) | ≤ 0.0005% |
| Sulfate (S04) | ≤0.05% |
Material is of non-animal, non-human origin
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References
1 N.E. Good, et al., Biochemistry, 5, 467 (1966).
